The seasonality of 10 of the most abundant species of macromycetes was studied at 3 sites in west-central Mexico. Collections over 20 years from the Instituto de Botánica of the Universidad de Guadalajara database were used for this study. Two of the sites were pine-oak forests: Mazamitla (70 collections) and Tapalpa (68 collections). The third consisted of an ecotone between subtropical cloud forest and pine-oak forest: Nevado de Colima (91 collections). The 3 sites are close to the tropical region and have a rainy season between late May and October. The development and abundance of fruit bodies were related to the climate and the vegetation of each site. Differences were found at 3 scales for both fructification time and abundance: between sites, between species, and within species at different sites.